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24, chemin de Borde Rouge –Auzeville – CS52627
31326 Castanet Tolosan CEDEX - France

Dernière mise à jour : Mai 2018

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Lipoprotein and polysaccharide-protein structures (ELIPS)

Victory is in the kitchen

              Context and Objectives - Research lines - Methods and Equipment

                          Recent work - Patents ans Thesis - Publications


Team leader: Benedicte BAKAN

Permanent team members:

Researchers and engineers:  Bénédicte Bakan, Khalil Elmorjani, Didier Marion

Technicians and assistant engineers:  Nathalie Geneix, André Lelion,  Anne-Lyse Panheleux

Temporary staff members:

Nicolas Reynoud (PhD) Mathilde Marc (CDD engineer)


Nicolas Moreau (  ) Coralie Prin (Bachelor's degree in apprenticeship), Hélène Février (CDD engineer), Jessica Laot (CDD engineer)

Thesis defended recently:

Glenn Philippe (2017),  Nathalie Anciaux-Geneix (2016), Mathieu Gayral (2015)


Context and Objectives

Processing and nutritional quality of plant products depends in part on the assembly of the various components within the plant organs harvested. In this context we focus specifically on the one hand to the structure and assembly of the cuticles of fruits (including caryopses of cereals) and secondly, to the formation of amyloid-protein matrix grains cereals. Our goal is to characterize the major cellular, molecular and physicochemical mechanisms accompanying the formation of these supramolecular plants to identify new markers closely combining plant breeding and technology food or non-food processing.


Research lines

  • Formation and structure of cuticles: relationship with the processing and quality of plant products
  • Formation et structure of the amyloid-protein matrix grain: relationship with the processing of cereals


Methods et Equipment

  • Analysis and purification of proteins and lipids: 1D and 2D electrophoresis, HPLC analytical and semi-preparative reversed phase and normal phase, ion exchange chromatography and size exclusion, affinity chromatography, gas chromatography coupled with mass spectrometry, HPLC coupled to mass spectrometry.
  • Cloning, PCR, production and purification of recombinant proteins
  • Immunochemical methods (ELISA, immunoblotting) and immunocytochemistry (coupled with confocal microscopy, transmission electron microscopy)
  • Structural analysis: circular dichroism, fluorescence spectroscopy and infrared


Recent work

  • Highlighting the role of GDSL-lipase/acylhydrolase in the formation of cutin of tomato fruit
  • Puroindolines and hardness of wheat: New Perspectives
  • Towards sustainable processes in malting



Protein, fat, cuticle, cereals, fruits, development, aggregation, polymerization interactions, biosynthesis