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24, chemin de Borde Rouge –Auzeville – CS52627
31326 Castanet Tolosan CEDEX - France

Dernière mise à jour : Mai 2018

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Helsens Nicolas

Team APPIFish

nicolas helsens
© NH

PhD Student

 Oniris site de la Chantrerie, CS40706, 44307 Nantes, France
 team APPIFish, building G5 2nd floor

Tel: 02 40 68 76 79

Nicolas Helsens has joined BIOEPAR and SECALIM . His thesis topic is "Characterization of bacterial communities and antibiotic resistance genes in farmed fish", in the framework of the Foodresistome project (RFI). He is supervised by Catherine Magras (SECALIM thesis director), Ségolène Calvez (BIOEPAR) and Hervé Prévost (SECALIM).

He will be with us from April 3, 2017 until March 31, 2020.

Summary of the thesis:

The acquisition of antibiotic resistance by bacteria is a growing phenomenon, with increasingly important health and economic impacts. The main factor involved is the use of antibiotics in human and animal health, particularly in France. They spread in the environment and cause the development of resistance in bacteria exposed to them.
Faced with this growing risk, the question arises as to the place of food as a source of transmission of antibiotic resistance genes. In particular, the aquaculture sector can be considered as an interface between the environment and the consumer, since the farming basins are exposed to the elements present upstream, in the waters of the rivers that feed them. This sector also has good societal acceptance, and aquaculture production in France reaches 42,000 tonnes annually (including 36,000 tonnes for rainbow trout alone).
In order to assess the link between antibiotic resistance and food, the FoodResistome project aims to study antibiotic residues and antibiotic resistance genes in bacterial communities present in a food, the rainbow trout fillet. This study will be conducted on fish at the end of the farming process and on the fish fillet obtained in processing plants.
This approach combining metagenomics, molecular biology and biochemistry techniques will provide a more accurate picture of the links between the nature and concentration of antibiotics, the biodiversity of bacterial communities and antibiotic resistance genes in the food matrix studied.

  • Helsens, N., Calvez, S., Bouju-Albert, A., Rossero, A., Prévost, H., and Magras, C. 2020. Comparison of Stomaching versus Rinsing for Recovering Bacterial Communities from Rainbow Trout (Oncorhynchus mykiss) Fillets. Journal of Food Protection, 83(9):1540-1546 [IF19=1.581] DOI: 10.4315/JFP-20-037.