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24, chemin de Borde Rouge –Auzeville – CS52627
31326 Castanet Tolosan CEDEX - France

Dernière mise à jour : Mai 2018

Menu Logo Principal Institut Agro Rennes-Angers Angers University   IRHS


BROUARD Nathalie


Technician Angers University
  • Contact

Equipe/Team STREMHO
42, rue Georges Morel
CS 60057
49071 BEAUCOUZE Cedex

Tel : +33 (0)2 41 22 56 00

Email :  

  • Skills and competences
  • Biologie moléculaire :

Extraction d’acides nucléiques (ADN, ARN) et analyse quantitative (Nanodrop)

Transcription reverse

Amplification génétique par PCR et q PCR

Electrophorèse d’acides nucléiques

  • Biochimie :

Analyses Immuno-enzymatique (Technique de l’ELISA), analyse proteique par Western-Blot

Dosage de H2O2 (technique par chemiluminescence)

Dosage de proteine (méthode de Bradford)

  • Biologie cellulaire végétale :

Isolement et cultures de protoplastes, de cellules, culture in-vitro de cellules, cals et plants

Travail en condition stérile, Transformation génétique de protoplastes

  • Chimie des solutions :

Préparation de solutions chimiques et organiques

Utilisation de produits CMR (Cancérigène, Mutagène, Reprotoxique)

Préparation de milieux de culture

  • Microscopie optique :

Observations en lumière blanche et fluorescente.

Mesures de caractéristiques cellulaires (taille, comptage de stomates)

Utilisation de logiciel d’analyse d’images (ZEISS : AxioVisio 3.1)

  • Culture et phénotypage de plantes :

Bouturage, empotage, suivi d’arrosage des essais plantes entières

Prélèvements et mesures d’organes, dissections fines (bourgeons) sous loupe

  • Expérimentations sur plantes :

Apports localisés de microvolumes de substances (hormones, sucres) sur organes de plantes

Prélèvements d’organes et conservation au froid

  • Physiologie végétale :

Mesures sur plantes de la conductance stomatique(poromètre), de la teneur en chlorophylle

See also

The other members of the team >>>