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Axes de recherche

Nos travaux de recherche sont structurés en 3 axes complémentaires :

• Cellules souches : identité, niche et réparation du muscle
• Mécanismes fondamentaux dans la maladie de Pompe
• Cross-talk motoneurone - fibre musculaire - cellule de Schwann dans la maladie de Pompe et l'Amyotrophie Spinale

Projets

H2020 FAIR CHARM

LYSAUMI

Projet de recherche partenariale sur la maladie de Pompe, financé par l'AFM-Téléthon et l'association Vaincre les Maladies Lysosomales.

 

ANR FishMuSC

Projet de recherche partenariale sur les mécanismes cellulaires et moléculaires du déclin de l'hyperplasie musculaire chez la truite, financé par l'ANR.   

Programme MuStem

Projet de recherche  translationnelle sur la qualification de la cellule MuStem comme agent thérapeutique pour le traitement des affections musculaires, financé par le FEDER.

 

Impact of FoxO3a overexpression on muscle pathophysiology in Pompe disease

Thèse                                    

Julien Pichon         

2017-2020

 

Local delivery of human MuStem cells into infarcted heart attenuates adverse tissue remodeling and preserves cardiac function

Thèse                                    

Alice Rannou

2016-2019

 

Muscle pathophysiology and involvement of satellite cells in Pompe disease

Thèse                                    

Lydie Lagalice

2015-2018

Multi-Harmonic nanoparticle as effective monitoring tool to track cells in tissue depth

 

Collaboration internationale          

2015-2017

 

Intrathecal gene therapy for moderate forms of spinal muscular atrophy

Thèse                                   

Karim Bey

2014-2017

 

Multiple mode of action of MuStem cells to induce muscle benefits on dystrophic context

Thèse   

Florence Robriquet                          

2013-2016

 

Gene therapy of the neurological manifestations of Pompe disease

Thèse                                    

Juliette Hordeaux

2010-2014

Long-term muscle repair and clinical efficacy in dystrophic dogs following transplantation of allogeneic MuStem cells

Thèse      

Thibaut Larcher & Benoît Fornasari   

2008-2011

 

Sélection de publications

2020

• Bey K., Deniaud J., Dubreil L., Joussemet B., Cristini J., Ciron C., Hordeaux J., Le Boulc'H M., Marche K., Maquigneau M., Guilbaud M., Moreau R., Larcher T., Deschamps J.-Y., Fusellier M., Blouin V., Sevin C., Cartier N., Adjali O., Aubourg P., Moullier P., Colle M.-A., Intra-CSF AAV9 and AAVrh10 Administration in Nonhuman Primates: Promising Routes and Vectors for Which Neurological Diseases? Molecular Therapy - Methods & Clinical Development, 2020, 17:771-784. DOI: 10.1016/j.omtm.2020.04.001.
• Rannou A., Toumaniantz G., Larcher T., Leroux I., Ledevin M., Hivonnait A., Babarit C., Fleurisson R., Dubreil L., Ménoret S., Anegon I., Charpentier F., Rouger K., Guével L., Human MuStem cell grafting into infarcted rat heart attenuates adverse tissue remodeling and preserves cardiac function hMuStem cells preserve function of infarcted heart.Molecular Therapy - Methods & Clinical Development, 2020, 18:446-463. DOI: 10.1016/j.omtm.2020.06.009.

2019

• Dubreil L., Lagalice L., Deniaud J., Sabourin A., Lovo C., Bey K., Hordeaux J., Thorin C., Sandt C., Jamme F., Colle M.-A., Assessment of adeno-associated virus gene therapies efficacy on acid alpha-glucosidase restoration and glycogen storage correction in cardiac muscle of Pompe disease mice using synchrotron infrared and ultraviolet microspectroscopies.Journal of Spectral Imaging, 2019, DOI: 10.1255/jsi.2019.a13.
• Larcher T., Fablet C., Renson P., Menard D., Hervet C., Saade G., Belloc C., Bourry O., Meurens F., Assessment of pulmonary tissue responses in pigs challenged with PRRSV Lena strain shows better protection after immunization with field than vaccine strains.Veterinary Microbiology, 2019,230:249-259. DOI: 10.1016/j.vetmic.2019.01.022.

2018

• Lagalice L., Pichon J., Gougeon E., Soussi S., Deniaud J., Ledevin M., Maurier V., Leroux I., Durand S., Ciron C., Franzoso F., Dubreil L., Larcher T., Rouger K., Colle M.-A., Satellite cells fail to contribute to muscle repair but are functional in Pompe disease (glycogenosis type II). Acta Neuropathologica Communications, 2018, 6(1):116. DOI: 10.1186/s40478-018-0609-y.
• Lorant J., Saury C., Schleder C., Robriquet F., Lieubeau-Teillet B., Négroni E., Leroux I., Chabrand L., Viau S., Babarit C., Ledevin M., Dubreil L., Hamel A., Magot A., Thorin C., Guevel L., Delorme B., Péréon Y., Butler-Browne G., Mouly V., Rouger K., Skeletal Muscle Regenerative Potential of Human MuStem Cells following Transplantation into Injured Mice Muscle. Molecular Therapy, 2018, 26(2):618-633. DOI: 10.1016/j.ymthe.2017.10.013.
• Saury C., Lardenois A., Schleder C., Leroux I., Lieubeau B., David L., Charrier M., Guével L., Viau S., Delorme B., Rouger K., Human serum and platelet lysate are appropriate xeno-free alternatives for clinical-grade production of human MuStem cell batches. Stem Cell Research Therapy, 2018, 9(1):128. DOI: 10.1186/s13287-018-0852-y.
• Dubreil L., Aviat F., Anthoine V., Ismail R., Rossero A., Federighi M., Confocal spectral microscopy-an innovative tool for tracking of pathogen agents on contaminated wooden surfaces. European Journal of Wood and Wood Products, 2018,76(3):1083-1085. DOI: 10.1007/s00107-018-1294-z.
• Lorant J., Larcher T., Jaulin N., Hedan B., Lardenois A., Leroux I., Dubreil L., Ledevin M., Goubin H., Moullec S., Deschamps J.-Y., Thorin C., André C., Adjali O., Rouger K., Vascular Delivery of Allogeneic MuStem Cells in Dystrophic Dogs Requires Only Short-Term Immunosuppression to Avoid Host Immunity and Generate Clinical / Tissue Benefits. Cell Transplantation, 2018, 27(7):1096-1110. DOI: 10.1177/0963689718776306.

2017

• Bey K., Ciron C., Dubreil L., Deniaud J., Ledevin M., Cristini J., Blouin V., Aubourg P., Colle M.-A., Efficient CNS targeting in adult mice by intrathecal infusion of single-stranded AAV9-GFP for gene therapy of neurological disorders.Gene Therapy, 2017, 24(5):325-332. DOI: 10.1038/gt.2017.18.
• Hordeaux J., Dubreil L., Robeveille C., Deniaud J., Pascal Q., Dequeant B., Pailloux J., Lagalice L., Ledevin M., Babarit C., Costiou P., Jamme F., Fusellier M., Mallem Y., Ciron C., Huchet C., Caillaud C., Colle M.-A., Long-term neurologic and cardiac correction by intrathecal gene therapy in Pompe disease. Acta Neuropathologica Communications, 2017, 5(1):66. DOI: 10.1186/s40478-017-0464-2.
• Dubreil L., Leroux I., Ledevin M., Schleder C., Lagalice L., Lovo C., Fleurisson R., Passemard S., Kilin V., Gerber-Lemaire S., Colle M.-A., Bonacina L., Rouger K., Multi-Harmonic Imaging in the Second Near-Infrared Window of Nanoparticle-Labeled Stem Cells as Monitoring Tool in Tissue Depth. ACS Nano, 2017. DOI: 10.1021/acsnano.7b00773.

2016

• Lardenois A., Jagot S., Lagarrigue M., Guével B., Ledevin M., Larcher T., Dubreil L., Pineau C., Rouger K., Guével L., Quantitative proteome profiling of dystrophic dog skeletal muscle reveals a stabilized muscular architecture and protection against oxidative stress after systemic delivery of MuStem cells. Proteomics, 2016, 16(14):2028-2042. DOI: 10.1002/pmic.201600002.
• Robriquet F., Babarit C., Larcher T., Dubreil L., Ledevin M., Goubin H., Rouger K., Guével L., Identification in GRMD dog muscle of critical miRNAs involved in pathophysiology and effects associated with MuStem cell transplantation. BMC Musculoskeletal Disorders, 2016,17(1):209. DOI: 10.1186/s12891-016-1060-5.

2015

• Hordeaux J., Dubreil L., Deniaud J., Iacobelli F., Moreau S., Ledevin M., Le Guiner C., Blouin V., Le Duff J., Mendes-Madeira A., Rolling F., Cherel Y., Moullier P., Colle M.-A., Efficient central nervous system AAVrh10-mediated intrathecal gene transfer in adult and neonate rats. Gene Therapy, 2015, 22(4):316-324. DOI: 10.1038/gt.2014.121.
• Robriquet F., Lardenois A., Babarit C., Larcher T., Dubreil L., Leroux I., Zuber C., Ledevin M., Deschamps J.-Y., Fromes Y., Cherel Y., Guével L., Rouger K., Differential Gene Expression Profiling of Dystrophic Dog Muscle after MuStem Cell Transplantation. PLoS ONE, 2015,10(5): e0123336. DOI: 10.1371/journal.pone.0123336.